Gene description for VTA1
Gene name vesicle trafficking 1
Gene symbol VTA1
Other names/aliases 6orf55
C6orf55
DRG-1
DRG1
HSPC228
LIP5
My012
SBP1
Species Homo sapiens
 Experiment description of studies that identified VTA1 in Colorectal cancer cells

1
Experiment ID303
Identified moleculemrna
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Microarray [Illumina]
PubMed ID 19930720   
OrganismHomo sapiens
Experiment descriptionColorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
AuthorsHong BS, Cho JH, Kim H, Choi EJ, Rho S, Kim J, Kim JH, Choi DS, Kim YK, Hwang D, Gho YS
Journal name BMC Genomics
Publication year2009
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methodsDifferential centrifugation
Ultrafiltration
OptiPrep density gradient
Flotation density1.09 g/mL
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-PCR
Microarray [Illumina]
EV-TRACK -

2
Experiment ID480
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDKO-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%

3
Experiment ID481
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDks-8
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%

4
Experiment ID482
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23161513   
OrganismHomo sapiens
Experiment descriptionProteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
AuthorsDemory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year2012
SampleColorectal cancer cells
Sample nameDLD-1
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130004: EV-METRIC:44%

5
Experiment ID517
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23585443   
OrganismHomo sapiens
Experiment descriptionProteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components.
AuthorsJi H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ.
Journal name Proteomics
Publication year2013
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130010: EV-METRIC:44%

6
Experiment ID518
Identified moleculeprotein
Extracellular vesicle typeExosomes/Membrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23585443   
OrganismHomo sapiens
Experiment descriptionProteome profiling of exosomes derived from human primary and metastatic colorectal cells reveal differential expression of key metastatic factors and signal transduction components.
AuthorsJi H, Greening DW, Barnes TW, Lim JW, Tauro BJ, Rai A, Xu R, Adda C, Mathivanan S, Zhao W, Xue Y, Xu T, Zhu HJ, Simpson RJ.
Journal name Proteomics
Publication year2013
SampleColorectal cancer cells
Sample nameSW620
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130010: EV-METRIC:44%

7
Experiment ID549
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles/Ectosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 24009881   
OrganismHomo sapiens
Experiment descriptionQuantitative proteomics of extracellular vesicles derived from human primary and metastatic colorectal cancer cells.
AuthorsChoi DS, Choi DY, Hong BS, Jang SC, Kim DK, Lee J, Kim YK, Kim KP, Gho YS.
Journal name J Extracell Vesicles
Publication year2012
SampleColorectal cancer cells
Sample nameSW480
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120013: EV-METRIC:50%

8
Experiment ID550
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles/Microvesicles/Ectosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 24009881   
OrganismHomo sapiens
Experiment descriptionQuantitative proteomics of extracellular vesicles derived from human primary and metastatic colorectal cancer cells.
AuthorsChoi DS, Choi DY, Hong BS, Jang SC, Kim DK, Lee J, Kim YK, Kim KP, Gho YS.
Journal name J Extracell Vesicles
Publication year2012
SampleColorectal cancer cells
Sample nameSW620
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120013: EV-METRIC:50%

9
Experiment ID589
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHCT-116
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

10
Experiment ID590
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleColorectal cancer cells
Sample nameHCT-15
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

11
Experiment ID696
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics
Publication year2017
SampleColorectal cancer cells
Sample nameLIM1215- insulin induced
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170021: EV-METRIC:44%, 44%

12
Experiment ID697
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics
Publication year2017
SampleColorectal cancer cells
Sample nameLIM1215- non-induced
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV170021: EV-METRIC:44%, 44%

13
Experiment ID1049
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleColorectal cancer cells
Sample name LIM1215 - NI
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV170021: EV-METRIC:44%, 44%

14
Experiment ID1050
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 28842968   
OrganismHomo sapiens
Experiment descriptionInsulin Mediated Activation of PI3K/Akt Signalling Pathway Modifies the Proteomic Cargo of Extracellular Vesicles.
AuthorsLiem M, Ang CS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleColorectal cancer cells
Sample namelim1215 - II
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV170021: EV-METRIC:44%, 44%

15
Experiment ID3338
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34887515   
OrganismHomo sapiens
Experiment descriptionSupermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
AuthorsZhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year2021
SampleColorectal cancer cells
Sample nameDiFi
Isolation/purification methodsDifferential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
Mass spectrometry
RNA sequencing
EV-TRACK -

16
Experiment ID3339
Identified moleculeprotein
Extracellular vesicle typeExomeres
MISEVEV-like nanoparticles
Identification method Mass spectrometry
PubMed ID 34887515   
OrganismHomo sapiens
Experiment descriptionSupermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
AuthorsZhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year2021
SampleColorectal cancer cells
Sample nameDiFi
Isolation/purification methodsDifferential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
Mass spectrometry
ELISA
RNA sequencing
EV-TRACK -

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